Enzymes {restriction enzyme} can cleave nucleotide sequences at sites. 150 enzymes {endonuclease}, such as FokI and NotI, cut DNA near 4-base to 8-base recognition sequences.
ends
Restriction enzymes can leave ends {blunt end} with paired bases or ends {sticky end} with overlaps. Sticky ends can bind to other sticky ends and then DNA ligase can seal them, allowing splicing with other DNA fragments. Blunt ends can become sticky by terminal transferase, which adds polyA or polyT to one strand. Blunt ends can become sticky by attaching a DNA linker, with recognition sites, to blunt ends and then cleaving with restriction enzyme.
middle
Endonucleases cut nucleic acids at sequence sites not at end. Pancreatic ribonuclease, T1 ribonuclease, and other ribonucleases cut only RNA. Bacterial restriction endonucleases and other deoxyribonucleases cut only double-stranded DNA.
S1 nuclease {exonuclease} pares back RNA and single-stranded DNA ends. Sticky ends can become blunt ends by removing single-stranded DNA using S1 nuclease.
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Date Modified: 2022.0225